Recombinant Human LILRB2/CD85d/ILT4 Domain1&2 Protein,His Tag

Description :
Source Recombinant Human LILRB2/CD85d/ILT4 Domain1&2 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Gln22-Val229.[Accession |Q8N423-1] Molecular Weight The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human LILRB2 Domain1&2, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA. ELISA Data: Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43g/ml. SPR Data: Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5M as determined in a SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.