Recombinant Human LILRB2/CD85d/ILT4 Protein,mFc Tag

Description :
Source Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminal. It contains Gln22-His458.[Accession |Q8N423-1] Molecular Weight The protein has a predicted MW of 73.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human LILRB2, mFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. ELISA Data: Serial dilutions of Anti-LILRB2 Antibody were added into Human HLA-G Complex Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.11g/ml. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.