Recombinant Human CD21 Protein,His Tag

Description :
Source Recombinant Human CD21 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ile21-Arg971.[Accession |P20023-1] Molecular Weight The protein has a predicted MW of 105.3 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Activity ELISA Data: Immobilized Human CD21, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.