Recombinant Human Glypican 1/GPC1 Protein,His Tag

Description :
Synonyms Glypican 1, GPC1 Uniprot No. P35052-1 Source Recombinant Human Glypican 1/GPC1 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Asp24-Ser530. Molecular Weight The protein has a predicted MW of 57.17 kDa. And it migrates as 60-72 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Purity > 90% as determined by SDS-PAGE. Biological Activity Human FGF-2 immobilized on CM5 chip can bind human GPC1, His tag, with an affinity constant of 5.83 10-9M as determined in a SPR assay (Biacore 8K). Endotoxin Formulation Lyophilized from a 0.2 m filtered concentrated solution in PBS. Reconstitution Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first usedand avoid repeated freeze-thaw cycles.