Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein,His-Avi Tag

Description :
Source Biotinylated Human Latent TGF beta 1/TGFB1 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Leu30-Ser390(C33S).[Accession |P01137] Molecular Weight The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 (LAP) /13-15 (Mature TGF beta 1) kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 93% as determined by HPLC Activity ELISA Data: Immobilized Human ITGAV&ITGB8, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.