Recombinant Human GAL1 Protein,hFc Tag

Description :
Synonyms S-Lac lectin 1; HLBP14;Galectin-1; GAL1 Source Human Galectin 1 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ala2-Asp135.[Accession | P09382] Molecular Weight The protein has a predicted MW of 41.9 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human Galectin 1, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-Galectin 1 Antibody, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.