Recombinant Mouse NGF Protein

Description :
Synonyms beta Nerve Growth Factor; Ngfb Uniprot No. NP_001106168.1 Source Recombinant Mouse NGF Proteinis expressed from CHO Stable CellsCellswith an initial Met at the C-terminus. It contains Ser 122-Gly 241. Molecular Weight The recombinant mouse NGF has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions. Biological Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50for this effect is 1-6 ng/mL. Purity > 95% as determined by SDS-PAGE. Endotoxin Formulation Lyophilized from sterile 150mM NaCl, 50mM NaAc, pH 5.5.Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectantsbefore lyophilization. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.