Recombinant Human ICOS/CD278 (Q50A) Protein,hFc Tag

Description :
Synonyms CRP-1;ICOS;CD278;AILIM Source Human ICOS/CD278 (Q50A) Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu21-Phe141.[Accession | Q9Y6W8-1] Molecular Weight The protein has a predicted MW of 40.45 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.