Recombinant Rat CD47 Protein,hFc Tag

Description :
Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 Source Rat CD47 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Gln19-Lys140.[Accession | P97829-1] Molecular Weight The protein has a predicted MW of 40.40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Mouse SIRP alpha, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.