Recombinant SARS-COV-2 Spike S1(Delta B.1.617.2) Protein, His Tag

Description :
别名 S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 Accession QHD43416.1 Recombinant SARS-CoV-2 Spike S1 (Delta B.1.617.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln14-Arg683(T19R, G142D, E156G, HR157-158 del, L452R, T478K, D614G, P681R). 分子量 Approximately 75.92 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. > 95% as determined by Tris-Bis PAGEand HPLC. ELISA DataImmobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) , His Tag at 0.5g/ml (100 l/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. 内毒素 Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.