Recombinant Human CD58 Protein,hFc Tag

Description :
Source Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Phe29-Arg215.[Accession |AAH05930] Molecular Weight The protein has a predicted MW of 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data: Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200). Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.