Recombinant Biotinylated Human IL-2R beta/CD122 Protein,His-Avi Tag

Description :
Source Biotinylated Human IL-2 R beta/CD122 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala27-Asp239.[Accession |NP_000869] Molecular Weight The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data1: Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 M as determined in SPR assay. SPR Data 2: Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined in SPR assay. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.